Wheat (Triticum aestivum L., cultivar 'Yecora rojo') was grown in ambi
ent (370 mu mol mol(-1)) or enriched (550 mu mol mol(-1)) concentratio
ns of CO2 in the free-air CO2 enrichment (FACE) project, and component
s were analyzed for in vitro digestibility, fiber constituents, and cr
ude protein. Four replicated plots of each CO2 treatment were split fo
r irrigation: 'wet' regions received 60 cm of water and 'dry' regions
received 30 cm of water through underground tubes. Enriched CO2 concen
trations had no effect on in vitro digestion of intact sections of you
ng (26-32-day-old plants) leaf blades except at 24-27 h incubation, at
which time enriched leaves were lower in digestibility than control o
nes. Enriched CO2 concentrations increased the content of acid deterge
nt fiber (ADF) and cellulose of young wet leaves, Sections of main sho
ots at 26 days tended to have increased digestibility with elevated CO
2 levels. Enriched CO2 concentrations did not alter the digestibility
of flag leaves from 105-day-old plants or of flag leaves, uppermost st
ems, and sheaths from plants at full grain maturity, Enriched CO2 leve
ls reduced the acid detergent lignin (ADL) and tended to reduce the pr
otein of leaves from 105-day-old plants. For mature leaf blades, neutr
al detergent fiber, ADF, and cellulose were, or tended to be, higher w
hile protein content tended to be lower in elevated CO2-grown plants;
for both CO2 treatments, 'dry' leaves were higher in digestibility and
lower in ADL than 'wet' samples. Mature stems plus sheaths had lower
protein contents in plants grown in elevated CO2. Results indicated th
at enriched CO2 concentrations to 550 mu mol mol(-1) did not substanti
ally alter wheat in vitro digestibility, regardless of irrigation trea
tment. Elevated CO2 altered fiber components and protein, but these we
re not consistent among parts and harvests.