BENEFICIAL EFFECT OF FRUCTOSE-1,6-BISPHOSPHATE ON MITOCHONDRIAL-FUNCTION DURING ISCHEMIA-REPERFUSION OF RAT-LIVER

Background/Aims: Several groups have reported that administration of f ructose-1,6-bisphosphate (FBP) reduces ischemic injury. The aim of thi s study was to determine the protective effect of FBP on the impairmen t of mitochondrial oxidative phosphorylation by ischemia-reperfusion i njury in the vat liver. Methods: The respiratory control ratio (RCR) a nd the adenine nucleotide content of mitochondria isolated from ischem ic and reperfused livers with or without FBP treatment were measured. Results: In FBP-treated livers, the cellular adenosine triphosphate le vel was Pestered to more than 50% of normal after 120 minutes of reper fusion following 120 minutes of ischemia, whereas that of control live rs only reached 15% of normal. The RCR and the adenine nucleotide cont ent of mitochondria isolated from FBP-treated livers were significantl y higher than those of mitochondria from control livers after ischemia and reperfusion. FBP strongly suppressed the formation of lipid perox ides during reperfusion. in vitamin E-deficient rats, the RCR decrease d markedly during reperfusion, but FBP protected the mitochondria agai nst reperfusion injury. Conclusions: FBP has a protective effect again st ischemia-reperfusion injury on the liver and especially preserves t he oxidative phosphorylation capacity of hepatic mitochondria.