Wn. Kuo et al., PROTEIN-KINASE C-EPSILON,THETA,IOTA IMMUNOREACTIVITY IN BAKERS-YEAST,LOBSTER AND WHEAT-GERM, Biochemical archives, 11(2), 1995, pp. 115-119
Varied patterns of immunoreactive bands of Ca2+-independent protein ki
nase C were detected by Western blotting. Intense wide bands, with spe
cific immunoreactivity to anti-PKCepsilon and anti-PKCiota, were found
in Active Dry and RapidRise yeasts. In comparison, separate bands wer
e observed in protease deficient yeast and wheat germ. The crude extra
cts from wheat germ, lobster tail meat and three strains of yeast disp
layed a well-defined 50-kD immounreactive band in the presence of anti
-PKCtheta.