ISOLATION OF RAT-LIVER TRNA(ASP), TRNA(CYS), TRNA(TYR), AND TRNA(VAL)ISOACCEPTOR SPECIES BY HPLC

Authors
KANDUC D BRACALELLO A MOSAVI A
Citation
D. Kanduc et al., ISOLATION OF RAT-LIVER TRNA(ASP), TRNA(CYS), TRNA(TYR), AND TRNA(VAL)ISOACCEPTOR SPECIES BY HPLC, Biochemical archives, 11(2), 1995, pp. 121-127
Citations number
10
Categorie Soggetti
Biology
Journal title
Biochemical archives → ACNP
ISSN journal
07495331
Volume
11
Issue
2
Year of publication
1995
Pages
121 - 127
Database
ISI
SICI code
0749-5331(1995)11:2<121:IORTTT>2.0.ZU;2-6
Abstract
Cytoplasmic tRNA population from rat liver has been fractionated by h. p.l.c. using a gradient of acetonitrile/ammonium acetate. Peak assignm ent and relative concentrations of iso-tRNAs for aspartic acid, cystei ne, tyrosine, and valine have been established. The relative concentra tions of these tRNA species have been expressed as area peak percentag es and compared to theoretical data for the fractional use of each cod on for aminoacid.