I. Junghahn et al., EFFECT OF ALP ANALOGS ON INOSITOL TRISPHOSPHATE FORMATION IN H183 MAMMARY EPITHELIAL-CELLS BEFORE AND AFTER TRANSFECTION WITH V-ERB-B ONCOGENE, Anticancer research, 15(2), 1995, pp. 449-454
The influence of cytostatically active alkyllysophospholipid analogs w
ith different chemical structure on IP3 (inositol-1,4,5-trisphosphate)
formation and intracellular Ca++ concentration was studied in human m
ammary epithelial cells before and after transfection with v-erb B onc
ogene DNA. Transformed cells showed an increased IP3 formation compare
d with normal cells. In the presence of ALP (alkyllysophospholipid) an
alogs IP3 formation in inhibited more strongly in transformed cells th
an in normal cells, dependent on the structure of ALP analogs. Further
more, these compounds increased [Ca++](i) (intracellular Ca++ concentr
ation) in transformed cells much more strongly than in normal cells. F
rom these results, obtained in pursuit of an oncogene-related cancer t
reatment, it follows that ALP analogs may inhibit processes in signal
transduction in cells more strongly after transfection with a defined
oncogene than before.