MAGNETIC-RESONANCE-IMAGING AS A MONITOR OF CHANGES IN THE IRRADIATED RAT-BRAIN - AN AID IN DETERMINING THE TIME-COURSE OF EVENTS IN A HISTOLOGIC-STUDY

RATIONALE AND OBJECTIVES. Optimal dose schedules and total dose of ion izing radiation for human central nervous system malignancy are not kn own. An animal model has been developed for the investigation of rat c entral nervous system response to proton irradiation using magnetic re sonance imaging (MRI). A clinical MRI device was used to monitor the r esponse of the rat brain after irradiation as a possible indicator for histologic injury as a function of time and dose. METHODS. Single-dos e fractions of protons were delivered to the left brain of 25 adult Sp raque-Dawley rats, T1- and T2-weighted images were obtained using a 1, 5-T MRI device via a 12-cm diameter coil at 4- to 6-week intervals aft er irradiation. Coronal images were evaluated by visual inspection and relaxation maps comparing the control and irradiated hemispheres. His tologic review was conducted on all rats' brains after death. RESULTS. Proton irradiation was delivered successfully to only the left brain of the animals. Histologic review confirms the location and extent of tissue damage demonstrated on MRIs obtained in vivo. Statistically sig nificant differences were seen in the T2-weighted relaxation times in the irradiated cerebral hemisphere compared with the unirradiated hemi sphere. CONCLUSION. The proton hemibrain rat model can be used to test treatment schedules of irradiation for central nervous system respons e using MRI to noninvasively document early and late effects within th e same animal.