P450(ALDO) IN HAMSTER ADRENAL-CORTEX - IMMUNOFLUORESCENT AND IMMONUGOLD ELECTRON-MICROSCOPIC STUDIES

Authors
LEHOUX JG MARTEL D LEHOUX J DUCHARME L LEFEBVRE A BRIERE N
Citation
Jg. Lehoux et al., P450(ALDO) IN HAMSTER ADRENAL-CORTEX - IMMUNOFLUORESCENT AND IMMONUGOLD ELECTRON-MICROSCOPIC STUDIES, Endocrine research, 21(1-2), 1995, pp. 275-280
Citations number
10
Categorie Soggetti
Endocrynology & Metabolism
Journal title
Endocrine research → ACNP
ISSN journal
07435800
Volume
21
Issue
1-2
Year of publication
1995
Pages
275 - 280
Database
ISI
SICI code
0743-5800(1995)21:1-2<275:PIHA-I>2.0.ZU;2-1
Abstract
The zonal distribution of aldosterone synthase cytochrome P450 (P450(a ldo)) in the adrenal cortex of male hamsters was investigated by immun ofluorescence and electron microscopy, using an anti-P450(aldo) peptid e antibody. On cryostat sections the immunolocalization of P450(aldo) was confined to the zona glomerulosa cells. On semi-thin plastic secti ons, P450(aldo) was shown to be located in mitochondria. Studies in el ectron microscopy, using the colloidal gold technique, confirmed that P450(aldo) was located in mitochondria.