METABOLISM OF 11-DEOXYCORTISOL BY CYTOCHROME P450(11-BETA) - IDENTIFICATION OF REACTION-PRODUCTS BY H-1-NMR AND LC MS-APCI METHOD/

Authors
OHTA M FUJII S NONAKA Y OKAMOTO M
Citation
M. Ohta et al., METABOLISM OF 11-DEOXYCORTISOL BY CYTOCHROME P450(11-BETA) - IDENTIFICATION OF REACTION-PRODUCTS BY H-1-NMR AND LC MS-APCI METHOD/, Endocrine research, 21(1-2), 1995, pp. 477-481
Citations number
5
Categorie Soggetti
Endocrynology & Metabolism
Journal title
Endocrine research → ACNP
ISSN journal
07435800
Volume
21
Issue
1-2
Year of publication
1995
Pages
477 - 481
Database
ISI
SICI code
0743-5800(1995)21:1-2<477:MO1BCP>2.0.ZU;2-Z
Abstract
A hitherto-unidentified steroid produced in the reaction mixture of 11 -deoxycortisol with bovine cytochrome P450(11 beta) reconstitution sys tem was purified by repeated HPLCs, and subjected to H-1-NMR analysis. The result suggested that the new metabolite was 19-oxo-11-deoxycorti sol. This was further confirmed by a new analytical technique using LC /MS equipped with atmospheric pressure chemical ion (APCI)-analyzer.