RECOGNITION ABILITY AND CYTOTOXICITY OF SOME OLIGOSACCHARIDYL SUBSTITUTED BETA-CYCLODEXTRINS

This paper reports a chemico-enzymatic synthesis of beta-CD derivative s. The recognition properties of these derivatives were tested using f locculating yeast and isolated lectins. It was observed that the subst itution of beta-cyclodextrins with galactose end arms induces the bett er recognition by a cell-linked galactose-specific lectin. The physico chemical effects of the beta-CD derivatives on membranes were estimate d using red blood cells and the effects on the viability of yeast and human rectal tumor cells were appreciated by measuring the mitochondri al deshydrogenase activity. The substitutions of the beta-CD ring by s ugar antennae decrease the negative physicochemical effects of the bet a-CD, ie their hemolytic properties. However, these substitutions indu ce significant modifications of the biological properties of the molec ules, particularly the cytotoxicity and the growth of eukaryotic cells .