EFFECT OF INTRALIPID(R) OVERDOSE ON BRAIN LIPID-COMPOSITION IN THE ADULT-RABBIT AND ITS RELATION WITH THE ROUTE OF ADMINISTRATION

A group of 33 New Zeland rabbits were injected with 4g/kg weight/day o f Intralipid (ITL) 20%. They were injected intravenously, intraperiton eally and intragrastically. The aim was to study the variations in pla sma and brain lipids after fats overdosages. A control group, which wa s operated without receiving ITL, was also established. After 15 days of daily Intralipid dosage, complex lipids and fatty acids (FA) admini stered with ITL rose in plasma levels in the intravenous and intraperi toneal groups. The study of brain lipids showed decreasing levels of p hosphatidilethanolamine (PhE), phosphatidilcholine and phosphatidilser ine (PhC+PhS), and palmitoil cerebroside (PC) in those groups parenter ally supplemented with ITL, without any variation in the polar lipds/n eutral lipids ratio (PL/NL). Those groups supplemented with ITL showed increasing levels of the stearic (C-18) and oleic (C-18:1) acids in b rain, whereas linoleic (C-18:2) and arachinodic (C-20:4) acids levels decreased. In conclusion parenteral Intralipid overdosages cause longe r-lasting changes in plasma lipids after intraperitoneal administratio n. A decreasing level of structural lipids, linoleic and arachidonic a cids and also the ratio between both is noticed in the brain affecting all supplemented groups. All these changes in the adult animals are d ifferent to the ones described in other animals and lactant humans.