MODULATION OF A COMPETITIVE N-METHYL-D-ASPARTATE RECEPTOR ANTAGONIST BINDING BY ZINC-OXIDE

Zinc through a zinc binding site is known to modulate the binding of a gonists at the NMDA receptor. In the present study, the ability of zin c oxide to alter the specific binding of [H-3]CGP-39653, a competitive NMDA receptor antagonist, was determined in homogenate of rat brain t issue. Analysis of saturation experiments indicated that zinc oxide si gnificantly increased the K-d without changing B-max of [H-3]CGP-39653 binding. Furthermore, the effect of ZnO on glutamate and glycine disp lacement of [H-3]CGP-39653 binding was determined. The results of the [H-3]CGP-39653 displacement study indicated that ZnO decreases the glu tamate and glycine displacement of [H-3]CGP-39653 binding.