RESPONSE OF A MURINE V-BETA-18-ALLOREACTIVE CTL CLONE TO BACTERIAL SUPERANTIGENS

We have isolated, by limiting dilution, a murine anti-H-2K(b) T cell l ine derived from AKR/J mouse spleen cells following stimulation with H -2K(b+) cells. This clone, designated 2AH10, has the phenotype H-2K(k), CD8(+), LFA-1(+), ICAM-1(+), and IL-2R(+) and, as determined by DNA -sequencing reactions, expresses the V beta 18 T cell receptor gene se gment. Clone 2AH10 specifically lysed H-2K(b+) target cells and on sti mulation with H-2K(b+) cells proliferated to secrete IFN-gamma, LT, an d GM-CSF. Clone 2AH10 was responsive to the bacterial superantigens SE C1 and TSST-1 when stimulated in the context of murine MHC class II+ c ells. In addition to SEC1 and TSST-1, when human MHC class II+ cells w ere employed, clone 2AH10 responded to a wider range of bacterial supe rantigens including SEE, SED, and SEE. (C) 1995 Academic Press, Inc.