GLYCOPROTEIN AND GANGLIOSIDE CHANGES IN HUMAN TROPHOBLASTS AFTER EXPOSURE TO PULSED DOPPLER ULTRASOUND

Changes in glycoprotein and ganglioside composition in human trophobla sts (eighth week of gestation) after in vitro exposure to pulsed Doppl er ultrasound (pulse duration 1.22 mu s; repetition frequency 11.1 kHz ; center frequency 4 MHz; ISPPA = 175.5 W/cm(2); ISPTA = 0.59 W/cm(2)) were investigated, Evacuated trophoblasts were divided in two halves and insonated for 10 min on top of a 6-cm layer of 5% gelatin in 50-mL tubes (Falcon) at 37 degrees C, One half of each trophoblast was sham insonated and served as an internal control, After insonation trophob lasts were maintained at 37 degrees C for 24 h, Glycoproteins were det ected using Lu-D-mannose specific lectins from Galanthus nivalis and N arcissus pseudonarcissus. A decrease in the expression of mannose cont aining glycoprotein mgp47 and an increase in expression of mgp54 were observed, Ganglioside composition was also significantly altered. Conc entrations of two gangliosides migrating similarly to GM2, and one sim ilarly to GQ1, decreased by more than 75%, At the same time, concentra tions of one ganglioside migrating similarly to GM3, and two other uni dentified gangliosides increased two- to fourfold.