Oaks (Quercus petrdea, Q. robur) from several sites in eastern Austria
affected by novel oak decline (NOD), as well as oaks (Q. robur) showi
ng symptoms of oak yellows (OY), were tested for the presence of mycop
lasma-like organisms (MLOs). The samples, comprising roots, annual sho
ots, and branches, were examined using light-, fluorescence, and elctr
on-microscopic techniques. Staining with the DNA-fluorochrome DAPI pro
vided no evidence for the presence of MLOs in any of the samples. Furt
hermore, no pathological sieve-tube necrosis and no abnormal callose a
ccumulation could be observed. An association of MLOs with 'novel oak
decline' or 'oak yellows' is, therefore, unlikely.