A PROTEASE-DEPENDENT TCR-INDUCED DEATH PATHWAY IN MATURE LYMPHOCYTES

Citation
A. Sarin et al., A PROTEASE-DEPENDENT TCR-INDUCED DEATH PATHWAY IN MATURE LYMPHOCYTES, The Journal of immunology, 154(11), 1995, pp. 5806-5812
Citations number
34
Categorie Soggetti
Immunology
Journal title
The Journal of immunology
ISSN journal
00221767 → ACNP
Volume
154
Issue
11
Year of publication
1995
Pages
5806 - 5812
Database
ISI
SICI code
0022-1767(1995)154:11<5806:APTDPI>2.0.ZU;2-C
Abstract
Several cysteine and serine protease inhibitors previously shown to bl ock TCR-induced death of 2B4 T hybridoma cells were tested for their a bility to block various T lymphocyte apoptotic death systems. TCR-indu ced death of both peripheral CD4(+) and CD8(+) T cell blasts was inhib ited similarly to the hybridoma, but cell death in these cells induced by anti-Fas, gamma-irradiation, etoposide, or extracellular ATP was n ot blocked. For T cell lines, cell death induced by CTL or by IL-2 wit hdrawal was also not inhibited. TCR-induced death oi immature CD4(-)8( +) thymocytes triggered by culture on immobilized anti-CD3 was not blo cked by these protease inhibitors, whereas similar death induced in th e resting CD4(+)8(-) thymocyte subset under these conditions was inhib ited similarly to the T cell blasts. TCR-induced proliferation of the latter subset was modest in the absence of oxogeneous IL-2, but was en hanced two- to fourfold by the protease inhibitors. These results show that a protease-dependent death pathway can be triggered by the TCR i n mature T cells; similar protease-dependent steps are not common to t he TCR-triggered activation pathway or other apoptotic death pathways in these cells.