BIOSYNTHESIS OF EXTRACELLULAR RIBONUCLEASE IN BACILLUS-PUMILUS

Biosynthesis of extracellular alkaline ribonucleases (RNases) in Bacil lus pumilus is studied and compared with the regularities of RNase bio synthesis in Bacillus intermedius. It was established that B. pumilus RNase is an enzyme of the second growth phase. Inorganic phosphate inh ibits while actinomycin D stimulates RNase biosynthesis both in B. pum ilus and B. intermedius. However, dynamics of enzyme accumulation, the degree of RNase biosynthesis inhibition with phosphate, and sporulati on conditions substantially differ in these cultures.