N-(2-CHLOROETHYL)-N-ETHYL-2-BROMOBENZYLAMINE (DSP-4) HAS DIFFERENTIALEFFICACY FOR CAUSING CENTRAL NORADRENERGIC LESIONS IN 2 DIFFERENT RATSTRAINS - COMPARISON BETWEEN LONG-EVANS AND SPRAGUE-DAWLEY RATS
Rj. Schuerger et Cd. Balaban, N-(2-CHLOROETHYL)-N-ETHYL-2-BROMOBENZYLAMINE (DSP-4) HAS DIFFERENTIALEFFICACY FOR CAUSING CENTRAL NORADRENERGIC LESIONS IN 2 DIFFERENT RATSTRAINS - COMPARISON BETWEEN LONG-EVANS AND SPRAGUE-DAWLEY RATS, Journal of neuroscience methods, 58(1-2), 1995, pp. 95-101
We tested the hypothesis that Long-Evans (LE) and Sprague-Dawley (SD)
rat strains were equally sensitive to the noradrenergic neurotoxin N-(
2-chloroethyl)-N-ethyl-2-bromobenzylamine (DSP-4) with respect to cent
ral lesions of locus coeruleus (LC) terminals as measured by immunohis
tochemical localization of dopamine-beta-hydroxylase (D beta H). Analy
sis of D beta H immunoreactivity was made by both qualitative and quan
titative methods. Intraperitoneal injections of 50 mg/kg DSP-4 caused
a dramatic reduction of noradrenergic terminals in the neocortex, hipp
ocampus and cerebellum of SD, but not LE rats as compared to saline-in
jected controls. This finding indicates that LE rats are less sensitiv
e than SD rats to the neurotoxic effects of DSP-4 in the central nervo
us system.