DEVELOPMENT OF MONOCLONAL-ANTIBODIES TO IRON-REGULATED OUTER-MEMBRANEPROTEINS AND LIPOPOLYSACCHARIDE OF AEROMONAS-SALMONICIDA

Two monoclonal antibodies (MAbs) were obtained against the iron-regula ted outer membrane proteins (IROMPs) and 4 MAbs were obtained against the lipopolysaccharide (LPS) of Aeromonas salmonicida, as demonstrated by immunoblotting. MAbs directed against LPS reacted with whole bacte ria, both in the enzyme linked immunosorbent assay (ELISA) and by the indirect fluorescent antibody technique (IFAT). Of the 2 MAbs directed against the IROMPs, one did not react with whole bacteria either by I FAT or by ELISA, whereas the other reacted weakly with whole bacteria in both the tests. Some potential applications of the MAbs, including screening of bacterial isolates, are also discussed.