STRATEGIES FOR THE SYNTHESIS AND SCREENING OF GLYCOCONJUGATES .2. COVALENT IMMOBILIZATION FOR FLOW-CYTOMETRY

Authors
VETTER D TATE EM GALLOP MA
Citation
D. Vetter et al., STRATEGIES FOR THE SYNTHESIS AND SCREENING OF GLYCOCONJUGATES .2. COVALENT IMMOBILIZATION FOR FLOW-CYTOMETRY, Bioconjugate chemistry, 6(3), 1995, pp. 319-322
Citations number
18
Categorie Soggetti
Biology,Chemistry
Journal title
Bioconjugate chemistry → ACNP
ISSN journal
10431802
Volume
6
Issue
3
Year of publication
1995
Pages
319 - 322
Database
ISI
SICI code
1043-1802(1995)6:3<319:SFTSAS>2.0.ZU;2-3
Abstract
Glycosylamines are readily available carbohydrate derivatives that und ergo acylation reactions with homobifunctional. N-hydroxysuccinimidyl esters. The product glycosylamides carry a spacer group equipped with one active ester functionality. This route provides well-defined glyco conjugates, which may be cross-linked to various amino-functionalized resins. Carbohydrate recognition of the resulting sugar-bead conjugate s is probed by lectin immunostaining or flow cytometry using a fluores cently labeled lectin.