G. Briganti et al., STRUCTURAL VARIATIONS INDUCED BY MAGNESIUM-IONS AND TEMPERATURE IN THE LARGE RIBOSOMAL-SUBUNIT FROM THE EXTREME THERMOPHILE SULFOLOBUS-SOLFATARICUS, Nuovo cimento della Societa italiana di fisica. D, Condensed matter,atomic, molecular and chemical physics, biophysics, 16(9), 1994, pp. 1573-1577
Small-angle neutron scattering (SANS) measurements on the large 50S ri
bosomal subunit of the extreme thermophile archaebacterium Sulfolobus
solfataricus are presented. The structural effects induced by magnesiu
m ions and temperature are investigated. Supramolecular aggregation wa
s present in all the investigated conditions. Removal of magnesium ion
s and increase of temperature reduce the intensity of the scattering a
t Q = 0. This reduction is discussed looking at the Fourier inversion
of the scattering intensity, which includes both inter- and intra-part
icle contributions.