IN-VITRO CULTURE USED FOR WOODY FERN CYATHEA-AUSTRALIS (R BR) DOMIN VEGETATIVE PROPAGATION

Citation
K. Goller et Jj. Rybczynski, IN-VITRO CULTURE USED FOR WOODY FERN CYATHEA-AUSTRALIS (R BR) DOMIN VEGETATIVE PROPAGATION, Acta Societatis Botanicorum Poloniae, 64(1), 1995, pp. 13-17
Citations number
NO
Categorie Soggetti
Plant Sciences
ISSN journal
00016977
Volume
64
Issue
1
Year of publication
1995
Pages
13 - 17
Database
ISI
SICI code
0001-6977(1995)64:1<13:ICUFWF>2.0.ZU;2-K
Abstract
Experiments have been carried out on vegetative multiplication of Cyat hea australis. Cultures were initiated from spores collected from spor angium of mature fronds. Spores were sterilized in 3% chloramine with Tween and sown on Anderson (1984) medium supplemented with 80.0 mg/l o f Ads and solidified by 8.0 g/l of agar. After three months of spore g ermination overgrowth of prothallia was observed. Multiplication of pr othallia was stimulated by MS (1962) medium supplemented with 0.25 mg/ l BAP, 0.50 mg/l IBA, 0.50 mg/l IAA, 1.00 mg/l GA(3), 40.00 mg/l Ads a nd sucrose 30.0 g/l. Small drops of water were placed on the basal par t of gametophyte in order to help ovary fertilization. After the next few weeks the first very fragile, small and green crosier emerged from the base of gametophyte. Perlite culture stimulated root formation an d plant hardening to soil conditions.