ULTRASENSITIVE BIOANALYTICAL ASSAYS USING TIME-RESOLVED FLUORESCENCE DETECTION

This article reviews the use of time-resolved fluorimetric detection o f lanthanide chelate luminescence as a detection method for ultrasensi tive bioanalytical assays. Assay formats and detection methods, and th e principle of time-resolved fluorimetric detection, are described. De tection systems, assay formats, reagents, and instrumentation for time -resolved fluorimetric detection are outlined. A review of published a nd commercially available immunoassays and DNA hybridization assays us ing time-resolved fluorimetric detection of lanthanide chelate lumines cence is given.