MOLECULAR-CLONING AND EXPRESSION OF CHICK GAL-BETA-1,3GALNAC ALPHA-2,3-SIALYLTRANSFERASE

A cDNA clone encoding chick Gal beta 1,3GalNAc alpha 2,3-sialyltransfe rase (ST3Gal I) was isolated from a chick embryo brain cDNA library. T he cDNA sequence included an open reading frame coding for 342 amino a cids, and the deduced amino acid sequence showed 64% identity with tha t of the mouse enzyme. Northern blot analysis of chick embryos reveale d that the ST3Gal I gene was expressed in early embryonic stages. The identity of the enzyme was confirmed by construction of a recombinant sialyltransferase in which the N-terminal part including the cytoplasm ic tail and signal anchor domain was replaced with an immunoglobulin s ignal peptide sequence. This enzyme expressed in COS-7 cells exhibited transferase activity similar to that of mouse ST3Gal I.