AMYLIN AND EPINEPHRINE HAVE NO DIRECT EFFECT ON GLUCOSE-TRANSPORT IN ISOLATED RAT SOLEUS MUSCLE

Amylin and epinephrine did not significantly affect insulin stimulated , or basal, 3-O-methylglucose transport in isolated rat soleus muscle, as measured by the release of 3-O-methylglucose from pre-loaded tissu e, Both amylin and epinephrine inhibited insulin-stimulated 2-deoxyglu cose uptake (by 25% and 38%, respectively) in soleus muscle from fed r ats but not from fasted rats, The latter results are consistent with a mylin and epinephrine stimulating glycogenolysis and inhibiting hexoki nase activity by intracellular accumulation of glucose 6-phosphate. We conclude that amylin, like epinephrine, does not specifically inhibit glucose transporters in skeletal muscle.