ISOLATION OF GENES FROM THE BATTEN CANDIDATE REGION USING EXON AMPLIFICATION

In order to identify genes originating from the Batten disease candida te region, we have used the technique of exon amplification to identif y transcribed sequences, This procedure produces trapped exon clones, which can represent single exons or multiple exons spliced together an d is an efficient method for obtaining probes for physical mapping and for screening cDNA libraries, The source of DNA for these experiments was a collection of chromosome 16 cosmid contigs isolated by the dire ct subcloning of region-specific yeast artificial chromosomes (YACs) a nd hybridization of inter-alu PCR products from these YACs to the flow -sorted Los Alamos chromosome 16 cosmid library. We are now using the resulting exon probes to screen retina and brain cDNA libraries for ca ndidate JNCL genes. (C) 1995 Wiley-Liss, Inc.