DISCRIMINATION OF THE NORTHEAST ATLANTIC LESSER SANDEELS, AMMODYTES MARINUS, AMMODYTES TOBIANUS AND GYMNAMMODYTES SEMISQUAMATUS BY PROTEIN ELECTROPHORESIS
Mj. Donaghy et al., DISCRIMINATION OF THE NORTHEAST ATLANTIC LESSER SANDEELS, AMMODYTES MARINUS, AMMODYTES TOBIANUS AND GYMNAMMODYTES SEMISQUAMATUS BY PROTEIN ELECTROPHORESIS, Fisheries research, 23(1-2), 1995, pp. 1-9
The northeast Atlantic lesser sandeels Ammodytes marinus Raitt, Ammody
tes tobianus L. and Gymnammodytes semisquamatus Jourdain were screened
by starch gel electrophoresis for species-specific enzyme variation.
Morphological separation of the genera is reliable but distinguishing
the two Ammodytes species is problematic, particularly in smaller spec
imens. The analysis revealed a number of diagnostic isozymes. Two, MEP
-1 and MEP-2, appear particularly suitable for discriminating the thre
e species, These isozymes show no intraspecific variation, can be type
d using muscle, are relatively stable in frozen tissue, and can be res
olved using a single gel and histochemical stain. The use of these dia
gnostic isozymes will facilitate species discrimination in fisheries a
ssessment work and in studies of the ecological differences among the
species.