2-STEPS PROCEDURE FOR THE PURIFICATION OF CORN FLOUR HYDROLYSATE

When corn flour hydrolysate filtrate (CFHF) was treated with 100% (w/v ) (NH4)(2)SO4 for purification, 80.5% of the protein was removed. Amon g the pH values studied, at pH 1.5, 90.2% of the total protein was pre cipitated from CFHF and supernatant was removed by decantation. The co rn flour hydrolysate was purified with Amberlite and DEAE-cellulose af ter precipitating the protein at pH 1.5. Charcoal was better than cati on- and anion-exchange resins for the removal of proteins, amino acids and pigments from the hydrolysate.