D-L AMINO-ACID-ANALYSIS USING AUTOMATED PRECOLUMN DERIVATIZATION WITH1-FLUORO-2,4-DINITROPHENYL-5-ALANINE AMIDE

An automated procedure for the precolumn derivatization of enantiomeri c amino acid mixtures with 1-fluoro-2,4-dinitrophenyl-5-alanine amide and a liquid chromatographic method for the separation of the derivati ves with UV detection are reported. The system described allows to per form routine analyses using microbore columns with a sensitivity at th e picomol level. Improvements for the use of this reagent in the proto col of a subtractive Edman degradation procedure of peptides to determ ine the sequence position of amino acid residues with concomitant iden tification of their chirality are also described.