SIMULTANEOUS DETERMINATION OF NADOLOL ENANTIOMERS IN HUMAN PLASMA BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY USING FLUORESCENCE DETECTION

A high-performance liquid chromatographic assay is described for the s eparation and quantification of nadolol isomers in human plasma, The i somers were quantified using reverse-phase HPLC and fluorometric detec tion after derivatization with the chiral reagent R(-)-1-(naphthyl)eth ylisocyanate [R(-)-NEI], The N-isopropyl analogue (one isomer) of nado lol was used as the internal standard. The method was reproducible bas ed on precision studies where the percent relative standard deviation was less than 15%, The lower limit of quantitation for each isomer was 2.5 ng/mL, This method was used to evaluate the pharmacokinetic profi le of nadolol isomers in human subjects following both single and mult iple oral dosing.