Ab. Orth et al., A SERINE (THREONINE) PROTEIN-KINASE CONFERS FUNGICIDE RESISTANCE IN THE PHYTOPATHOGENIC FUNGUS USTILAGO-MAYDIS, Applied and environmental microbiology, 61(6), 1995, pp. 2341-2345
A mutant of Ustilago maydis (VR43) with single-gene resistance to the
dicarboximide fungicide vinclozolin was previously isolated and charac
terized. A genomic library was constructed, and an 8.7-kb resistance-c
onferring fragment was isolated by sib selection. Sequencing this frag
ment, we identified an 1,218-bp open reading frame, which, if disrupte
d by deletion, no longer confers resistance. Analyses of the data in G
enBank demonstrated a high degree of homology between the product of t
he 1,218-bp open reading frame, referred to as the adr-1 gene, and Ser
(Thr) protein kinases.