A SERINE (THREONINE) PROTEIN-KINASE CONFERS FUNGICIDE RESISTANCE IN THE PHYTOPATHOGENIC FUNGUS USTILAGO-MAYDIS

Authors
ORTH AB RZHETSKAYA M PELL EJ TIEN M
Citation
Ab. Orth et al., A SERINE (THREONINE) PROTEIN-KINASE CONFERS FUNGICIDE RESISTANCE IN THE PHYTOPATHOGENIC FUNGUS USTILAGO-MAYDIS, Applied and environmental microbiology, 61(6), 1995, pp. 2341-2345
Citations number
22
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
Journal title
Applied and environmental microbiology → ACNP
ISSN journal
00992240
Volume
61
Issue
6
Year of publication
1995
Pages
2341 - 2345
Database
ISI
SICI code
0099-2240(1995)61:6<2341:AS(PCF>2.0.ZU;2-U
Abstract
A mutant of Ustilago maydis (VR43) with single-gene resistance to the dicarboximide fungicide vinclozolin was previously isolated and charac terized. A genomic library was constructed, and an 8.7-kb resistance-c onferring fragment was isolated by sib selection. Sequencing this frag ment, we identified an 1,218-bp open reading frame, which, if disrupte d by deletion, no longer confers resistance. Analyses of the data in G enBank demonstrated a high degree of homology between the product of t he 1,218-bp open reading frame, referred to as the adr-1 gene, and Ser (Thr) protein kinases.