XYLANASE PRODUCTION BY A HYPERXYLANOLYTIC MUTANT OF FUSARIUM-OXYSPORUM

Mutagenesis of Fusarium oxysporum DSM 841 enhanced the activity of xyl anase and beta-xylosidase enzymes by more than threefold. Wild-strain spores were subjected to UV or N-methyl-N'-nitro-N-nitrosoguanidine tr eatments. The hyperxylanolytic mutant (NTG-19) secretes high levels of xylanolytic enzymes on commercial xylan as well as several agricultur al residues, of which wheat bran supported maximum enzyme yields. Addi tion of 0.2% olive oil, an inexpensive source of fatty acids, resulted in a further 25% increase in enzyme secretion.