ACUTE TOXICITY OF CYCLOHEXYLMETHYLPHOSPHONOFLUORIDATE (CMPF) IN RHESUS-MONKEYS - SERUM BIOCHEMICAL AND HEMATOLOGIC CHANGES

Changes in serum biochemical and hematological parameters were studied in 20 male rhesus monkeys following acute poisoning by the organophos phate nerve agent cyclohexylmethylphosphonofluoridate (CMPF or GF). An imals were challenged with 5 x LD50 GF (233 mu g/kg, IM) following pre treatment with pyridostigmine (0.3-0.7 mg/kg per 24 h) and treated wit h atropine (0.4 mg/kg, IM) and either 2-PAM (25.7 mg/kg, IM) or HI6 (3 7.8 mg/kg, IM) at the onset of clinical signs or at 1 min after exposu re. Muscle fasciculations, tremors, or convulsions occurred in 19 of 2 0 animals. Serum biochemical and hematologic parameters were analyzed 2 days and 7 days after exposure and compared to pre-exposure baseline values. Significant increases in creatine kinase (CK), lactate dehydr ogenase (LD), aspartate transaminase (AST), alanine transaminase (ALT) and potassium ion (Kf), associated with damage to striated muscle and metabolic acidosis, occurred in both oxime-treated groups 2 days afte r exposure, Total protein, albumin, red blood cell (RBC) count, hemogl obin concentration (Hb) and hematocrit (Hct), were decreased in both o xime-treated groups at 7 days. The results demonstrate that animals ex posed to a single high dose of GF and treated with standard therapy ex hibit changes in serum biochemical and hematological indices directly and indirectly associated with their clinical presentations.