ROTAVIRUS DIARRHEA IN THAI INFANTS AND CHILDREN

Citation
Mk. Hendricks et al., ROTAVIRUS DIARRHEA IN THAI INFANTS AND CHILDREN, Annals of tropical paediatrics, 15(2), 1995, pp. 147-152
Citations number
NO
Categorie Soggetti
Pediatrics,"Tropical Medicine
ISSN journal
02724936
Volume
15
Issue
2
Year of publication
1995
Pages
147 - 152
Database
ISI
SICI code
0272-4936(1995)15:2<147:RDITIA>2.0.ZU;2-5
Abstract
One hundred and three children aged from 1 month to 5 years were asses sed for human rotavirus (HRV) infection over a 5-week period at the Ch ildren's Hospital, Bangkok. HRV was present in 18% and adenovirus in 1 0.7% of the children with gastro-enteritis. None of the controls excre ted HRV. Vomiting and dehydration were significantly associated with H RV diarrhoea. A comparison of the reliability of the detection methods showed the following sensitivities: RNA-polyacrylamide gel electropho resis (PAGE) 90%, electron microscopy (EM) 84% and Rotascreen latex pa rticle agglutination (LPA) 80%. PAGE and EM were 100% specific while t he Rotascreen LPA was 81% specific. Rotascreen LPA gave 15.5% false po sitive results. Of the HRV positive samples analyzed by PAGE, 5.3% and 94.7% were of subgroups I and II, respectively. The ID enzyme immunoa ssay and PAGE are reliable and inexpensive methods which can be recomm ended for HRV detection, particularly in areas with little technical s upport.