MEKC ANALYSIS OF FITC AND DTAF AMINO-ACID DERIVATIVES WITH LIF DETECTION

Authors
LALLJIE SPD SANDRA P
Citation
Spd. Lalljie et P. Sandra, MEKC ANALYSIS OF FITC AND DTAF AMINO-ACID DERIVATIVES WITH LIF DETECTION, Chromatographia, 40(9-10), 1995, pp. 513-518
Citations number
32
Categorie Soggetti
Chemistry Analytical
Journal title
Chromatographia → ACNP
ISSN journal
00095893
Volume
40
Issue
9-10
Year of publication
1995
Pages
513 - 518
Database
ISI
SICI code
0009-5893(1995)40:9-10<513:MAOFAD>2.0.ZU;2-A
Abstract
The application of micellar electrokinetic chromatography to the analy sis of complex mixtures of FITC and DTAF amino acid derivatives was st udied. Resolution optimization was attempted for a mixture of 21 amino acids. In the case of FITC, by increasing the analysis temperature to 35 degrees C, a total of 17 FITC amino acids could be separated. On t he other hand, by using a combination of mixed micelles (SDS/Brij-35) and the addition of organic modifiers, a total of 19 DTAF derivatized amino acids could be separated.