Fj. Brockman et al., EFFECT OF GASEOUS NITROGEN AND PHOSPHORUS INJECTION ON IN-SITU BIOREMEDIATION OF A TRICHLOROETHYLENE-CONTAMINATED SITE, Journal of hazardous materials, 41(2-3), 1995, pp. 287-298
Methane and air were injected through a horizontal well into a trichlo
roethylene-contaminated site at a depth of 160ft below ground surface
to stimulate methanotrophic biodegradation of trichloroethylene (TCE).
Sediment samples were analyzed after 35 weeks of methane and air inje
ction, and after 13 weeks of methane and air injection supplemented wi
th injection of the gases nitrous oxide and triethyl phosphate. Methan
otroph most-probable-number (MPN) values were very low in most of the
samples prior to the addition of nitrogen and phosphorus to the site,
and increased several orders of magnitude following the addition, Simi
larly, the frequency of TCE biodegradative potential in methanotrophic
enrichments increased approximately three orders of magnitude after t
he addition of nitrogen and phosphorus to the site. The MPN and biodeg
radative potential data indicated that the zone of influence after the
addition of nitrogen and phosphorus extended to at least 60 ft from t
he injection well in both the vertical and horizontal directions.