Z. Jianmin et al., ANALYSIS OF FUNCTIONAL EPITOPES ON THE DENGUE-2 ENVELOPE (E) PROTEIN USING MONOCLONAL IGM ANTIBODIES, Archives of virology, 140(5), 1995, pp. 899-913
Forty-two hybridomas secreting IgM antibody against dengue virus were
derived from spleen cells of dengue 2 infected mice. Antibody from 27
of these recognised the E protein of this virus. Of the 22 antibodies
which neutralised dengue 2, only two cross-reacted with other flavivir
uses. These 22 antibodies recognised three discrete domains on dengue
virions. Competitive binding studies with IgG monoclonal antibodies su
ggested that two of the three domains were recognised by both IgG and
IgM antibodies and that there were two additional domains which may be
recognised exclusively by either IgG or IgM antibodies. IgM antibodie
s reacting with domains recognised by IgG antibodies that enhanced inf
ection of susceptible cells by dengue 2, had no enhancing properties.
None of the IgM antibodies activated the serum complement system after
reacting with dengue 2 virions.