EUKARYOTIC DNA-REPLICATION

Recent discoveries suggest that the initiation of eukaryotic DNA repli cation involves at least two steps - one occuring near the completion of mitosis and the other at the onset of S phase - that bring about th e ordered assembly of initiator proteins at the origin. The identifica tion and characterization of components involved in promoting or antag onizing each of these steps has provided a preliminary understanding o f how replication initiation is regulated so precisely during the cell cycle.