PLANTLET REGENERATION FROM MATURE EMBRYOS OF JUNIPERUS-CEDRUS

A protocol is described for plantlet regeneration using embryonic expl ants of Juniperus cedrus Webb and Berth. An average of 6 adventitious buds were induced on whole excised embryos cultured for 15 days on Quo irin and LePoivre (QP) half-strength medium supplemented with 5 mu M N -6-benzyladenine. For bud development, explants were transferred to ph ytohormone-free 1/2 QP medium. For shoot elongation, explants were cul tured on 1/2 QP with 0.05% activated charcoal and 2% sucrose. Adventit ious shoots were rooted successfully in pear-vermiculite-perlite(1:1:1 ) moistened with 1/4 QP containing 1% sucrose and 5 mu M alpha-napthal eneacetic acid, pH 5.0. Axillary shoots elongated spontaneously in cul ture from leaf axils.