3,4-DICHLOROANILINE N-GLUCOSYLTRANSFERASE AND N-MALONYLTRANSFERASE ACTIVITIES IN CELL-CULTURES AND PLANTS OF SOYBEAN AND WHEAT

The activities of UDP-glucose: 3,4-dichloroaniline N-glucosyltransfera se (N-GT; EC 2.4.1.71) and malonyl-CoA: 3,4-dichloroaniline, N-malonyl transferase (N-MT) were determined in soybean and wheat cell suspensio n cultures, and in individual organs of the respective plants. In the soybean suspensions, N-GT and N-MT activities were quite constant over the subculture interval, and ranged from 0.48 to 1.65, and 34.3 to 80 .1 pkat . mg(-1) protein, respectively. No N-MT activity was detected in wheat cultures, while the specific N-GT activities were between 1.3 and 4.5 pkat . mg(-1) protein. In soybean plants, N-MT was most activ e in the roots (9.6 pkat . mg(-1) protein) and N-GT activity was maxim al in the cotyledons (1.7 pkat . mg(-1) protein). No N-MT activity was detected in the wheat plants, whereas N-GT activities of the wheat ro ots and cotyledons amounted to 1.4 and 1.0 pkat . mg(-1) protein, resp ectively.