A LINKAGE MAP FOR SUGI (CRYPTOMERIA-JAPONICA) BASED ON RFLP, RAPD, AND ISOZYME LOCI

A linkage map for sugi was constructed on the basis of restriction fra gment length polymorphism (RFLP), random amplified polymorphic DNA (RA PD), and isozyme loci using a three-generation pedigree prepared for g enetic analysis of heartwood color. A total of 128 RFLP (123 cDNA and 5 genomic probes), 33 RAPD, 2 isozyme, and 1 morphological (dwarf) loc i segregated in 73 progeny. Of the 164 segregating loci, 145 loci were distributed in 20 linkage groups. Of these loci, 91 with confirmed ma p positions were assigned to 13 linkage groups, covering a total of 88 7.3 cM. A clustering of markers with distorted segregation was observe d in 6 linkage groups. In the four clusters, distortions with a reduct ion in the number of homozygotes from one parent only were found.