GIBBERELLIN-REGULATED EXPRESSION IN OAT ALEURONE CELLS OF 2 KINASES THAT SHOW HOMOLOGY TO MAP KINASE AND A RIBOSOMAL-PROTEIN KINASE

cDNA fragments from ten different protein kinases expressed in Avena s ativa aleurone cells were amplified from mRNA by RT-PCR with degenerat e primers. These could be classified into five-groups: Aspk1-3 showed homology to the Snf1-related protein kinases, Aspk4-5 to a wheat ABA u p-regulated protein kinase, Aspk6-8 to the Ca-dependent, calmodulin-in dependent protein kinase family, Aspk9 encoded a MAP kinase and Aspk10 was closely related to a novel Arabidopsis ribosomal protein kinase. GA caused a rapid increase in transcripts hybridising to Aspk10, while inhibiting the dramatic accumulation of transcripts hybridising to As pk9 that occurred in the absence of GA.