USE OF IMMOBILIZED LIPASE IN TRANSESTERIFICATION OF TRIGLICERIDES

The lipase of Rhizopus origin has been used in immobilized enzyme form for hydrolysis and transesterification of triglycerides. The hydrolyt ic and transesterification activities of the enzyme fixed on Doulite E S 568, celite, cyclodextrin polymer, diatomaceous silica carriers were determined. The fatty acid composition of bioconversion products has been analyzed with Chrom 5 gas chromatograph and with Beckman HPLC app aratus. The multiple use of the immobilized enzyme is highly economica l. Keeping the optimal temperature, pH value and reaction time, the mo dification of triglycerides becomes a controllable biotechnological pr ocedure.