Experiment 1 measured the effect of different amounts of dietary fish
meal on milk yield and composition. Milk fat percentage and yield were
decreased by increased fish meal intake, but this change was not asso
ciated with changes in ruminal fermentation patterns. plasma long-chai
n n-3 polyunsaturated fatty acids were increased as intake of fish mea
l increased. Ruminal disappearance of DM, CP, and lipid in fish meal w
as measured in situ. Seventy percent of lipid disappeared by 8 h. Intr
aruminal administration of fish oil did not alter ruminal fermentation
and only slightly changed fatty acid profiles in duodenal digesta, pl
asma, or milk. Duodenal infusion increased plasma n-3 fatty acids but
did not affect composition of fatty acids in milk. Experiment 2 compar
ed effects of dietary fish meal and fish oil on milk production and co
mposition. Fish meal increased n-3 fatty acids in plasma compared with
those of the fish oil treatment. No changes were found in milk yield
or composition because of experimental treatments. Cows fed fish meal
or fish oil differed significantly in plasma fatty acid profiles but d
id not differ in ruminal VFA concentrations or milk fat yield.