DETECTION OF SPECIFIC T-CELL REACTIVITY IN SHEEP INFECTED WITH MYCOBACTERIUM-AVIUM SUBSPECIES SILVATICUM AND PARATUBERCULOSIS USING 2 DEFINED MYCOBACTERIAL ANTIGENS

Authors
BURRELLS C INGLIS NF DAVIES RC SHARP JM
Citation
C. Burrells et al., DETECTION OF SPECIFIC T-CELL REACTIVITY IN SHEEP INFECTED WITH MYCOBACTERIUM-AVIUM SUBSPECIES SILVATICUM AND PARATUBERCULOSIS USING 2 DEFINED MYCOBACTERIAL ANTIGENS, Veterinary immunology and immunopathology, 45(3-4), 1995, pp. 311-320
Citations number
32
Categorie Soggetti
Immunology,"Veterinary Sciences
Journal title
Veterinary immunology and immunopathology → ACNP
ISSN journal
01652427
Volume
45
Issue
3-4
Year of publication
1995
Pages
311 - 320
Database
ISI
SICI code
0165-2427(1995)45:3-4<311:DOSTRI>2.0.ZU;2-R
Abstract
A 30 kDa antigen (P30) from Mycobacterium avium ssp. paratuberculosis (M. a. paratuberculosis) and a 40 kDa (P40) antigen from Mycobacterium avium ssp. silvaticum (M. a. silvaticum) were employed in two differe nt assays to measure the cell-mediated immune reactivity of ovine peri pheral blood lymphocytes. In lymphocyte stimulation assays, proliferat ive responses to the P30 were observed only with lymphocytes from shee p inoculated with live M. a, paratuberculosis or M. a. silvaticum. Alt hough this antigen was not subspecies-specific it differentiated betwe en animals given live organisms and those inoculated with an inactive lysate. The P40 protein from M. a. silvaticum showed subspecies specif icity by eliciting in vitro responses only with lymphocytes derived fr om sheep inoculated with live M. a. silvaticum. Similar results were o btained using an interferon-gamma release assay which proved to be a m ore rapid and sensitive system.