ANTIGEN DIFFUSION FROM THE PERILYMPHATIC SPACE OF THE COCHLEA

The diffusion pattern of horseradish peroxidase (HRP) injected into th e scala tympani of the cochlear basal turn of guinea pigs was studied to test whether antigen presented in this manner can gain access to th e endolymphatic sac. By two hours, HRP reaction product was found thro ughout the cochlea, with the greatest amounts in the spiral ligament, spiral limbus, basilar membrane, and organ of Corti. In several cochle as, very weak labeling was seen in the stria vascularis. HRP reaction product was maximal in the basal turn. By two hours, HRP reaction prod uct was also observed in the endolymphatic sac lumen, epithelial cells , subepithelial tissue, and perisaccular connective tissue. It was mor e common in the proximal portion. At this time, macrophages within the lumen already appeared to have phagocytosed the HRP. By 72 hours afte r injection, the inner ear was cleared of HRP. The results of this stu dy support the hypothesis that antigen in the scala tympani gains acce ss to the endolymphatic sac lumen, where it may be presented by macrop hages to the systemic immune system. Antigen most likely does not gain access to the endolymphatic space in the cochlea, but it gets to the endolymphatic sac through the perilymph and the perisaccular tissue.