A study was performed on freshwater gastrotrichs living in the interst
itial habitat of some lakes in Latium and of Lake Garda (Italy). Thirt
y-two chetonotids were collected, twenty-five of which could be identi
fied. Four are new to science (Chaetonotus (Hystricochaetonotus) benac
ensis, Heterolepidoderma lamellatum, Ichthydium plicatum, l. squamiger
um), and two are new to the Italian fauna (Heterolepidoderma macrops,
H. majus). The faunistic composition of the lakes is compared with pre
vious data. Interstitial gastrotrichs are less varied and abundant tha
n those of epibenthos and periphyton, but often are of taxonomical and
zoogeographical interest. The presence in the interstitial of some ep
ibenthic and periphytic species demonstrates their capacity to coloniz
e ecologically different habitats. The grain size of the substrate was
not clearly correlated with faunal richness, but there was a possible
influence of the amount of the organic detritus in the sediment. Morp
hological analysis showed a prevalence of small sized animals, with po
orly ornamentated cuticular covering and well developed locomotory cil
iature, as probable adaptations to interstitial life.