CHARACTERISTICS OF LIPASE MODIFIED WITH WATER-SOLUBLE ACYLATING REAGENTS AND ITS ESTERIFICATION ABILITY

Lip was modified with various hydrophobic groups, such as Z, Boc, laur oyl, and palmitoyl, using water-soluble acylating reagents, The chemic al modification enhanced the dispersibility of Lip in organic solvents , and changed the substrate selectivity, which depends on the length o f the carbon chain of triglycerides in hydrolytic reactions, As for th e synthesis of triglycerides, Z-modified Lip catalyzed this reaction f aster than unmodified Lip, Z-, Boc-, and fatty acid-modified Lips also catalyzed diverse esterification reactions including that of ethyl ei cosapentaenoate, lactose oleate, and cholesterol oleate, and the yield s of these reactions increased depending on the carbon number of the i ntroducing group.