THE COMPLETE AMINO-ACID-SEQUENCE OF CHITINASE-B FROM THE LEAVES OF POKEWEED (PHYTOLACCA-AMERICANA)

Authors
TANIGAWA M YAMAGAMI T FUNATSU G
Citation
M. Tanigawa et al., THE COMPLETE AMINO-ACID-SEQUENCE OF CHITINASE-B FROM THE LEAVES OF POKEWEED (PHYTOLACCA-AMERICANA), Bioscience, biotechnology, and biochemistry, 59(5), 1995, pp. 841-847
Citations number
16
Categorie Soggetti
Biology,Agriculture,"Biothechnology & Applied Migrobiology","Food Science & Tenology
Journal title
Bioscience, biotechnology, and biochemistry → ACNP
ISSN journal
09168451
Volume
59
Issue
5
Year of publication
1995
Pages
841 - 847
Database
ISI
SICI code
0916-8451(1995)59:5<841:TCAOCF>2.0.ZU;2-2
Abstract
The complete amino acid sequence of pokeweed leaf chitinase-B (PLC-B) has been determined by first sequencing all 19 tryptic peptides derive d from the reduced and S-carboxymethylated (RCm-) PLC-B and then conne cting them by analyzing the chymotryptic peptides from three fragments produced by cyanogen bromide cleavage of RCm-PLC-B. PLC-B consists of 274 amino acid residues and has a molecular mass of 29,473Da, Six cys teine residues are linked by disulfide bonds between Cys20 and Cys67, Cys50 and Cys57, and Cys159 and Cys188, From 58-68% sequence homology of PLC-B with five class III chitinases, it was concluded that PLC-B i s a basic class III chitinase.