STIMULATORY FACTORS FOR ENZYMATIC BIOTIN SYNTHESIS FROM DETHIOBIOTIN IN CELL-FREE-EXTRACTS OF ESCHERICHIA-COLI

Authors
OHSHIRO T YAMAMOTO M BUI BTS FLORENTIN D MARQUET A IZUMI Y
Citation
T. Ohshiro et al., STIMULATORY FACTORS FOR ENZYMATIC BIOTIN SYNTHESIS FROM DETHIOBIOTIN IN CELL-FREE-EXTRACTS OF ESCHERICHIA-COLI, Bioscience, biotechnology, and biochemistry, 59(5), 1995, pp. 943-944
Citations number
14
Categorie Soggetti
Biology,Agriculture,"Biothechnology & Applied Migrobiology","Food Science & Tenology
Journal title
Bioscience, biotechnology, and biochemistry → ACNP
ISSN journal
09168451
Volume
59
Issue
5
Year of publication
1995
Pages
943 - 944
Database
ISI
SICI code
0916-8451(1995)59:5<943:SFFEBS>2.0.ZU;2-F
Abstract
The activity of biotin synthesis from dethiobiotin was found in cell-f ree extracts of an Escherichia coli bioB transformant. Among the sulfu r compounds tested, only S-adenosyl-L-methionine (AdoMet) had a signif icant effect, while methionine and cysteine were inert. The activity w as linearly stimulated by increasing protein concentration, When the d ialyzed cell-free extracts were used for the reaction, NADP(+), NADPH, and FAD among the well-known cofactors tested promoted the activity. Furthermore, in the presence of AdoMet, cysteine was apparently effect ive for biotin synthetic activity.