Cell population sociology was studied in two medulloblastomas and 10 a
strocytic human tumor cell Lines by means of the characterization of t
he structure of neoplastic cell colonies growing on histological slide
s, This was carried out via digital cell image analysis of Feulgen-sta
ined nuclei, to which the Delaunay triangulation and Voronoi paving ma
thematical techniques were applied, Such assessments were compared to
the DNA ploidy level (assessed by means of DNA histogram typing), The
results show that the cell colony architecture characteristics differe
d markedly according to whether the cell Lines were euploid (diploid o
r tetraploid) or aneuploid (hyperdiploid, triploid, hypertriploid, or
polymorphic), In fact, the cell colonies from the euploid cell nuclei
populations were larger and more dense than those from the aneuploid o
nes. Furthermore, for an identical period of culture, the cell lines f
rom high-grade malignant astrocytic tumors (glioblastomas) exhibited c
ell colonies that were larger and more dense than those in cell Lines
from low-grade astrocytic tumors (astrocytomas), In each of these two
groups, the diploid cell nuclei populations exhibited cell colonies la
rger and more dense than the nondiploid colonies, The present methodol
ogy is now being applied in vivo to histological sections of surgicall
y removed human brain tumors in order to distinguish between high-risk
clinical subgroups and medium-risk subgroups in clearly circumscribed
histopathological groups. (C) 1995 Wiley-Liss, Inc.